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human immortalized normal prostate epithelial pnt2 cells  (ATCC)


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    ATCC human immortalized normal prostate epithelial pnt2 cells
    Human Immortalized Normal Prostate Epithelial Pnt2 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+immortalized+normal+prostatic+epithelial+pnt2+cells/Primary+Prostate+Epithelial+Cells%3B+Normal+Human/pm39805041__ac4c04134_si_001-43-0-10
    Average 94 stars, based on 47 article reviews
    human immortalized normal prostate epithelial pnt2 cells - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Construct:

    Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.. The cells were grown at 37 °C in a humidified incubator with 5% CO 2 .The cells were grown at 37 °C in a humidified incubator with 5% CO 2 .

    Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml 1 penicillin and 100 mg ml 1 streptomycin.. The cells were grown at 37 C in a humidied incubator with 5% CO2.The cells were grown at 37 C in a humidied incubator with 5% CO2.

    Article Title: Determination of the glycoprotein specificity of lectins on cell membranes through oxidative proteomics
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells and human prostate carcinoma epithelial cells LNCaP were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin.. The cells were maintained in a humidified incubator at 37 °C with 5% CO 2 and subcultured at 80% confluency.The cells were maintained in a humidified incubator at 37 °C with 5% CO 2 and subcultured at 80% confluency.

    Article Title: Determination of the glycoprotein specificity of lectins on cell membranes through oxidative proteomics
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells and human prostate carcinoma epithelial cells LNCaP were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) penicillin.. Cells were maintained in a humidified incubator at 37 °C with 5% CO2 and subcultured at 80% confluency.Cells were maintained in a humidified incubator at 37 °C with 5% CO2 and subcultured at 80% confluency.

    Glycoproteomics:

    Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.. The cells were grown at 37 °C in a humidified incubator with 5% CO 2 .The cells were grown at 37 °C in a humidified incubator with 5% CO 2 .

    Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml 1 penicillin and 100 mg ml 1 streptomycin.. The cells were grown at 37 C in a humidied incubator with 5% CO2.The cells were grown at 37 C in a humidied incubator with 5% CO2.

    Article Title: Determination of the glycoprotein specificity of lectins on cell membranes through oxidative proteomics
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells and human prostate carcinoma epithelial cells LNCaP were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin.. The cells were maintained in a humidified incubator at 37 °C with 5% CO 2 and subcultured at 80% confluency.The cells were maintained in a humidified incubator at 37 °C with 5% CO 2 and subcultured at 80% confluency.

    Article Title: Determination of the glycoprotein specificity of lectins on cell membranes through oxidative proteomics
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells and human prostate carcinoma epithelial cells LNCaP were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) penicillin.. Cells were maintained in a humidified incubator at 37 °C with 5% CO2 and subcultured at 80% confluency.Cells were maintained in a humidified incubator at 37 °C with 5% CO2 and subcultured at 80% confluency.

    Binding Assay:

    Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.. The cells were grown at 37 °C in a humidified incubator with 5% CO 2 .The cells were grown at 37 °C in a humidified incubator with 5% CO 2 .

    Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml 1 penicillin and 100 mg ml 1 streptomycin.. The cells were grown at 37 C in a humidied incubator with 5% CO2.The cells were grown at 37 C in a humidied incubator with 5% CO2.

    Article Title: Determination of the glycoprotein specificity of lectins on cell membranes through oxidative proteomics
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells and human prostate carcinoma epithelial cells LNCaP were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS) and 1% (v/v) penicillin.. The cells were maintained in a humidified incubator at 37 °C with 5% CO 2 and subcultured at 80% confluency.The cells were maintained in a humidified incubator at 37 °C with 5% CO 2 and subcultured at 80% confluency.

    Article Title: Determination of the glycoprotein specificity of lectins on cell membranes through oxidative proteomics
    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells and human prostate carcinoma epithelial cells LNCaP were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) penicillin.. Cells were maintained in a humidified incubator at 37 °C with 5% CO2 and subcultured at 80% confluency.Cells were maintained in a humidified incubator at 37 °C with 5% CO2 and subcultured at 80% confluency.



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    ATCC human immortalized normal prostatic epithelial pnt2 cells
    The protein interaction networks revealed from the <t>PNT2</t> cell line. (a) The network was constructed based on the glycoproteins (red) interacting with other proteins through their glycans. The size of the circle is proportional to the number of proteins involved in the interaction. (b) A network corresponding to a protein that interacted with glycoproteins through other protein glycans. The glycan linkage was annotated based on Hex ( n ) HexNAc ( n ) Fuc ( n ) Sia ( n ) and colored as in the legend.
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    Image Search Results


    The protein interaction networks revealed from the PNT2 cell line. (a) The network was constructed based on the glycoproteins (red) interacting with other proteins through their glycans. The size of the circle is proportional to the number of proteins involved in the interaction. (b) A network corresponding to a protein that interacted with glycoproteins through other protein glycans. The glycan linkage was annotated based on Hex ( n ) HexNAc ( n ) Fuc ( n ) Sia ( n ) and colored as in the legend.

    Journal: Chemical Science

    Article Title: Glycan–protein cross-linking mass spectrometry reveals sialic acid-mediated protein networks on cell surfaces †

    doi: 10.1039/d1sc00814e

    Figure Lengend Snippet: The protein interaction networks revealed from the PNT2 cell line. (a) The network was constructed based on the glycoproteins (red) interacting with other proteins through their glycans. The size of the circle is proportional to the number of proteins involved in the interaction. (b) A network corresponding to a protein that interacted with glycoproteins through other protein glycans. The glycan linkage was annotated based on Hex ( n ) HexNAc ( n ) Fuc ( n ) Sia ( n ) and colored as in the legend.

    Article Snippet: Human immortalized normal prostatic epithelial PNT2 cells were obtained from ATCC and grown in Roswell Park Memorial Institute (RPMI) 1640 medium, while supplemented with 10% (v/v) fetal bovine serum (FBS), 100 U ml −1 penicillin and 100 μg ml −1 streptomycin.

    Techniques: Construct, Glycoproteomics